Photodynamic antimicrobial therapy (PACT) offers a promising alternative to traditional antibiotics. This work evaluates chlorin‑e6 (Ce6) encapsulated in PLGA nanoparticles against multidrug‑resistant Acinetobacter baumannii. Nanocarriers (~150 nm) exhibited ~80% Ce6 encapsulation efficiency.
Under 660 nm LED light (50 J/cm²), PACT achieved ≥99.9% bacterial inactivation within 15 minutes in planktonic cultures. Biofilm viability was reduced by >90%, confirmed by XTT assays and fluorescence microscopy. ROS detection assays demonstrated singlet oxygen production.
Cytotoxicity assays using human dermal fibroblasts indicated >80% viability at treatment concentrations. In a murine burn wound model inoculated with A. baumannii, treatment led to a >3 log₁₀ reduction in CFU and accelerated wound healing without histological tissue damage. These findings support PLGA-Ce6-PDT as a potent, localized antimicrobial strategy.
